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Microbial Genetics

Corsi di laurea:
Molecular Biology and Genetic
Docenti:
Calvio Cinzia
Anno accademico:
2010/2011
Crediti formativi:
6
Decreto Ministeriale:
270/04
Ore di lezione:
48
Lingua di insegnamento:
Italiano

ModalitÓ

Esame orale

Prerequisiti

Nessuno

Programma

1) Bacterial Genetics Analysis
a) Definitions, phenotypes, the pre-adaptive origin of mutations: Fluctuation test, Newcombe experiment, Replica plating, Sib selection
b) Isolating mutants, complementation and recombination tests, genetic crosses, gene knock-out, phenotypic or segregation lag.
2) Plasmids
a) Replication, copy number regulation, segregation and incompatibility.
3) Competence and transformation
a) Discovery of the main steps
b) Competence in Gram- and in Gram+
c) Competence regulation in B. subtilis: Quorum sensing and signal transduction, comK expression and transcriptional regulation
4) Conjugation
a) Mechanisms of transfer of the F conjugative plasmid, identification of tra genes, Hfr strains, F’ plasmids and their selection
b) Conjugation in Gram+
c) Discovery of conjugation
d) Construction of genetic maps by conjugation
e) Conjugative transposons and ICE
5) Lytic bacteriophages
a) Lytic cycle, transcriptional regulation in T7 and T4
b) Phage replication: RNA phages, filamentous phages, M13, T7, T4
c) Phage lysis
d) Genetic analysis of phages: Recombination and complementation tests, Benzer and T4 r mutants, Crick and the genetic code,
e) Generalized transduction
6) Lysogeny and phage
a) Mechanisms governing the lytic and lysogenic cycles: Induction, replication, lysogen formation, integration, immunity, CI activities
b) Mutants: CI, CII, CIII, vir, zygotic induction, nut mutants isolation
c) Specialized transduction, pbio and dgal formation and excision
d) Other lysogens: P2, P4, P1, SaPI1?. Addiction systems, morons
7) Transposons
a) Types,
b) Transposases: Replicative or Conservative mechanisms of transposition
c) Site specific recombination: Recombinases, Integrons, genetic islands, phase variation
8) Homologous recombination
a) Models, enzymes, phage enzymes
b) Genetic analysis of recombiantion
c) Gene conversion, High negative interference
9) Dna Repair
a) RecA? and the SOS regulon: Genetic identification of genes for the mutagenic repair pathway
10) Regulation of gene expression
a) The gal and trp operons in E.coli, the L-ara operon and the trp operon in B. subtilis
b) Riboswitch regulation
11) Regulons and stimulons
a) Catabolite repression in E. coli & B. subtilis
b) The heat shock response in E. coli & B. subtilis
c) The general stress response in E. coli & B. subtilis
d) Envelope stress responses
e) Regulation of Ribosome and tRNA synthesis
12) Developmental regulation in B. subtilis
a) Sporulation
b) Biofilm formation
c) Phenotypic heterogeneity

Bibliografia

L. Snyder & W. Champness
Molecular Genetics of Bacteria, 3rd Edition.
ASM Press


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